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KMID : 0350519920450041389
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Journal of Catholic Medical College
1992 Volume.45 No. 4 p.1389 ~ p.1403
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Demonstration of Angiogenic Activity from Rabbit Ocular Tissues by Rabbit Corneal Micropcket Assay
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Abstract
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The mechanism of retinal neovascularization remains unknown. It has been suggested that a vasoformative substance liberated by ischemic retina or the altered metabolic pathways or by-products of such altered metabolism of hypoxic retinal cells
might be
the cause of retinal neovascularization.
Further evidence suggesting that a retinal derived angiogenic substance has a role in the ocular disorders under discussion stems from the observation that destruction of retinal tissue results in the regression of the neovascularization.
In the present study, it was attempted to demonstrate an angiogenic activity from rabbit ocular tissues according to the age and the effect of panretinal photocoagulation (PRP) by rabbit corneal micropocket technique.
Tissue extracts of retina, vitreous, and choroid from newborn(20 eyes) and adult(28 eyes) rabbits were used. Adult rabbits were divided into 2 groups, with and without PRP.
Corneal microphotography was performed on both eyes of each animal on days 5, 9, 15, and 21 after operation to make records and pormit quantitative assessment of neovascularization with morphometry.
@ES The results were as follows:
@EN 1. Choroid showed the most appreciable capability of angiogenic activity; however, no significant difference could be found among those three groups.
2. There was a vasoproliferative substance in retina and its potency was higher in newborn rabbit than in two group of adult rabbits(P<0.05).
3. Regarding to the animal age, the angiogenic activity of vitreous decreased markedly with growing up, whereas the decrease in retina with animal age was less significant(P<0.001).
4. PRP appeared to reduce slightly the expression of angiogenic activity, but did not act directly in eliminating angiogenic activity in retina.
5. There was a correlation between the area of neovascularization and the protein of molecular weight 14.4KD(P<0.001).
6. PRP did not affect the absorbance of proteins in retina, but it caused decrease of proteins with molecular weight 22.5KD and 31 KD and increase of proteins with molecular weight 66.2KD in vitreous.
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